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esp3i restriction sites  (Addgene inc)


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    Structured Review

    Addgene inc esp3i restriction sites
    Esp3i Restriction Sites, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/esp3i+restriction+sites/pTE4398+(Plasmid+%2374042)/pm40328761-350-25-35
    Average 93 stars, based on 13 article reviews
    esp3i restriction sites - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Structural and functional insights into the interaction between Ku70/80 and Pol X family polymerases in NHEJ
    Article Snippet: The gap-filling reporter substrate was assembled into the pEGFP-N1 vector (Clontech) following sequential insertion of various PCR products, oligonucleotide linkers and synthetic DNA fragments (see Fig. for a detailed description). .. The Cpf1 and guide RNA co-expression plasmid used to cleave the reporter substrate was generated by inserting the pre-annealed gRNA-GF-F and gRNA-GF-R oligonucleotides into the Esp3I restriction sites of pTE4398 (a gift from Ervin Welker; Addgene plasmid # 74042; http://n2t.net/addgene:74042 ; RRID:Addgene_74042). .. The pNLS-mTagBFP2 plasmid used as an internal control of transfection efficiency was obtained by PCR amplification of the 2xNLS-mTagBFP2 coding sequence with mTagBFP-Acc65-F and mTagBFP-Mlu-R primers on the pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2 plasmid template (a gift from Thoru Pederson; Addgene plasmid # 64512; http://n2t.net/addgene:64512 ; RRID:Addgene_64512).

    Article Title: Structural and functional insights into the interaction between Ku70/80 and Pol X family polymerases in NHEJ.
    Article Snippet: The gap-filling reporter substrate was assembled into the pEGFPN1 vector (Clontech) following sequential insertion of various PCR products, oligonucleotide linkers and synthetic DNA fragments (see Fig. 4a for a detailed description). .. The Cpf1 and guide RNA coexpression plasmid used to cleave the reporter substrate was generated by inserting the pre-annealed gRNA-GF-F and gRNA-GF-R oligonucleotides into the Esp3I restriction sites of pTE4398 (a gift from Ervin Welker; Addgene plasmid # 74042; http://n2t.net/addgene: 74042; RRID:Addgene_74042). .. The pNLS-mTagBFP2 plasmid used as an internal control of transfection efficiency was obtained by PCR amplification of the 2xNLS-mTagBFP2 coding sequence with mTagBFP-Acc65-F and mTagBFP-Mlu-R primers on the pHAGE-TO-nls-st1dCas9-3nls3XTagBFP2 plasmid template (a gift from Thoru Pederson; Addgene plasmid # 64512; http://n2t.net/addgene:64512; RRID:Addgene_64512).

    Generated:

    Article Title: Structural and functional insights into the interaction between Ku70/80 and Pol X family polymerases in NHEJ
    Article Snippet: The gap-filling reporter substrate was assembled into the pEGFP-N1 vector (Clontech) following sequential insertion of various PCR products, oligonucleotide linkers and synthetic DNA fragments (see Fig. for a detailed description). .. The Cpf1 and guide RNA co-expression plasmid used to cleave the reporter substrate was generated by inserting the pre-annealed gRNA-GF-F and gRNA-GF-R oligonucleotides into the Esp3I restriction sites of pTE4398 (a gift from Ervin Welker; Addgene plasmid # 74042; http://n2t.net/addgene:74042 ; RRID:Addgene_74042). .. The pNLS-mTagBFP2 plasmid used as an internal control of transfection efficiency was obtained by PCR amplification of the 2xNLS-mTagBFP2 coding sequence with mTagBFP-Acc65-F and mTagBFP-Mlu-R primers on the pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2 plasmid template (a gift from Thoru Pederson; Addgene plasmid # 64512; http://n2t.net/addgene:64512 ; RRID:Addgene_64512).

    Article Title: Structural and functional insights into the interaction between Ku70/80 and Pol X family polymerases in NHEJ.
    Article Snippet: The gap-filling reporter substrate was assembled into the pEGFPN1 vector (Clontech) following sequential insertion of various PCR products, oligonucleotide linkers and synthetic DNA fragments (see Fig. 4a for a detailed description). .. The Cpf1 and guide RNA coexpression plasmid used to cleave the reporter substrate was generated by inserting the pre-annealed gRNA-GF-F and gRNA-GF-R oligonucleotides into the Esp3I restriction sites of pTE4398 (a gift from Ervin Welker; Addgene plasmid # 74042; http://n2t.net/addgene: 74042; RRID:Addgene_74042). .. The pNLS-mTagBFP2 plasmid used as an internal control of transfection efficiency was obtained by PCR amplification of the 2xNLS-mTagBFP2 coding sequence with mTagBFP-Acc65-F and mTagBFP-Mlu-R primers on the pHAGE-TO-nls-st1dCas9-3nls3XTagBFP2 plasmid template (a gift from Thoru Pederson; Addgene plasmid # 64512; http://n2t.net/addgene:64512; RRID:Addgene_64512).



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